Summaries by Inventor Shen, Bang

Plasmids for Cryptosporidium parvum
Funkhouser-Jones, Lisa ; Shen, Bang ; Sibley, Laurence
T-018967

— These plasmids are for performing CRISPR/Cas9 gene editing in Cryptosporidium parvum To generate a CRISPR/Cas9 plasmid for use in C. parvum, researchers used restriction cloning with SacI to insert the C. parvum U6 gene into a pUC19 vector to create pUC19-CpU6. We then inserted the C. parvum actin …